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Following the diffusion of cryopreservatives in tissues by stimulated Raman scattering microscopy (Conference Presentation)
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Paper Abstract

Cryopreservatives like dimethyl sulfoxide and glycerol are common agents that prevent cellular damage upon freezing of tissues or entire organisms. Although the cryopreservation capabilities of these compounds have been known empirically for years, much is unknown about the actual perfusion and distribution of the agents within cells on the microscopic scale. In this contribution, we report on studies that aim to uncover the dynamic distribution of cryopreservatives in the tissue with the aid of stimulated Raman scattering microscopy, enabling a direct and real-time view of the cellular loading and accumulation dynamics of these agents at the micrometer scale.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088227 (4 March 2019); doi: 10.1117/12.2510523
Show Author Affiliations
Richard C. Prince, Univ. of California, Irvine (United States)
Eric O. Potma, Univ. of California, Irvine (United States)
Nancy Levinger, Colorado State Univ. (United States)

Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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