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Measuring sub-nanometre thickness changes during phase transitions of supported lipid bilayers with quantitative differential interference contrast microscopy
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Paper Abstract

Quantitative differential interference contrast (qDIC) microscopy is applied to the study of the main phase transition of dipentadecanoylphosphatidylcholine (DC15PC) supported lipid bilayers. We measure thickness changes of about 1nm occurring in the bilayer with sub-nanometre resolution and show how the presence of fluorescently labelled lipids, even at small concentrations, can broaden the phase transition.

Paper Details

Date Published: 4 March 2019
PDF: 7 pages
Proc. SPIE 10887, Quantitative Phase Imaging V, 1088719 (4 March 2019); doi: 10.1117/12.2510461
Show Author Affiliations
David Regan, Cardiff Univ. (United Kingdom)
Joseph Williams, Cardiff Univ. (United Kingdom)
Francesco Masia, Cardiff Univ. (United Kingdom)
Paola Borri, Cardiff Univ. (United Kingdom)
Wolfgang Langbein, Cardiff Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 10887:
Quantitative Phase Imaging V
Gabriel Popescu; YongKeun Park, Editor(s)

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