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Pseudo-volumetric fluorescence endomicroscopy with a fiber bundle (Conference Presentation)
Author(s): Jean-Marc Tsang; Jason Ritt; Jerome Mertz

Paper Abstract

The development of high resolution, 3D imaging endomicroscopy has recently been gaining interest due to needs in in vivo studies of neuronal dynamics and interactions in deep brain tissue. However, most types of endomicroscopy techniques adopting micro-objectives can hardly perform 3D imaging due to the lack of minimally invasive scanning mechanism. Here, we present a GRIN-lens based fluorescence endomicroscopy capable of obtaining pseudo 3D image of a fluorescent sample without scanning the probe. We use a confocal laser scanning microscope (CLSM) to scan the proximal end of a coherent fiber bundle and obtain an extended depth of focus at the distal end. Preliminary results using fluorescent beads showed that a single bead can be detected throughout hundreds of microns depth. In the detection path, we use two reflecting pinholes of different size to form two confocal and one non-confocal images of the proximal end of the fiber bundle - one pinhole conjugates to the single core being illuminated and the other pinhole conjugates to the surrounding cores. The intensity ratio of an object in these three images almost explicitly depends on its distance from the distal end of the fiber bundle. Thus, we can determine the relative depths of objects in a volume sample in a single acquisition. Future works will include calibrating the setup to obtain the true depth instead of relative depth and applying this technique to brain samples.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10854, Endoscopic Microscopy XIV, 1085411 (4 March 2019); doi: 10.1117/12.2510407
Show Author Affiliations
Jean-Marc Tsang, Boston Univ. (United States)
Jason Ritt, Boston Univ. (United States)
Jerome Mertz, Boston Univ. (United States)

Published in SPIE Proceedings Vol. 10854:
Endoscopic Microscopy XIV
Guillermo J. Tearney M.D.; Thomas D. Wang; Melissa J. Suter, Editor(s)

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