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Label-free volumetric quantitative imaging of human osteosarcoma cells by hyperspectral coherent anti-Stokes Raman scattering
Author(s): Arnica Karuna; Francesco Masia; Marie Wiltshire; Rachel Errington; Paola Borri; Wolfgang Langbein
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Paper Abstract

Quantitative determination of the chemical composition of unstained samples, non-invasively, with high three- dimensional spatio-temporal resolution, will accelerate progress in cell biology. The current state of the art in bioimaging is dominated by either chemically non-specific or invasive methods. In this work, we demonstrate label-free, non-invasive quantitative volumetric imaging of human osteosarcoma cells using coherent anti-Stokes Raman scattering microscopy. A data analysis method developed in-house was applied to represent the chemical composition of the cells as volumetric three-dimensional images indicating water, proteins, DNAP (mixture of DNA and proteins), and lipids, and to determine the dry masses of the organic components with picogram resolution.

Paper Details

Date Published: 22 February 2019
PDF: 10 pages
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821P (22 February 2019); doi: 10.1117/12.2510277
Show Author Affiliations
Arnica Karuna, Cardiff Univ. (United Kingdom)
Delft Univ. of Technology (Netherlands)
Francesco Masia, Cardiff Univ. (United Kingdom)
Marie Wiltshire, Cardiff Univ. (United Kingdom)
Rachel Errington, Cardiff Univ. (United Kingdom)
Paola Borri, Cardiff Univ. (United Kingdom)
Wolfgang Langbein, Cardiff Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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