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Ultra-broad tunable low noise multi-color light source for multi-photon imaging and spectroscopy (Conference Presentation)
Author(s): Heiko Linnenbank; Tobias Steinle; Florian Mörz; Moritz Floess; Francisco Robles; Stefan Kedenburg; Harald Giessen
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Paper Abstract

We present an ultra‐low noise, near to mid infrared light source for a variety of multiphoton imaging and spectroscopy techniques. The system is based on an optical parametric oscillator (OPO) pumped by a femtosecond Ytterbium solid state oscillator with tens of megahertz repetition rate. This light source supplies three intrinsically synchronized light beams at wavelengths: 1040 nm, 1400-2000 nm (tunable) and 2200-4200 nm (tunable). Without active stabilization, the OPO preserves the shot-noise limited performance of the Yb-oscillator, along with a high long-term stability and a TEM00 beam profile. While this tuning range is already suitable for two- and three-photon microscopy, it now becomes possible to address vibrational modes and thus molecular specificity by employing further frequency conversion stages. Tailored frequency doubling provides either a narrow linewidth (0.5-1.2 nm) or a broadband (>40 nm) beam, tunable from 750-950 nm. The fixed Stokes beam of the Yb-oscillator can directly be used as a pump source for coherent Raman scattering such as SRS or CARS spectroscopy/microscopy. To this end, we will demonstrate the capability of our system for both SRS imaging at video rate with a spectral precision of 13 cm-1 as well as SRS spectroscopy with more than 400 cm-1 bandwidth in a single shot. By further mixing the two output beams of the OPO, we are able to additionally produce mid-infrared light that is tunable from 4-16 µm. With the help of vibrational sum frequency generation, our system will allow us to cover a spectral range of 700-7000 cm-1.

Paper Details

Date Published: 4 March 2019
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Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108820V (4 March 2019); doi: 10.1117/12.2510197
Show Author Affiliations
Heiko Linnenbank, Stuttgart Instruments GmbH (Germany)
Univ. Stuttgart (Germany)
Tobias Steinle, Univ. Stuttgart (Germany)
Florian Mörz, Stuttgart Instruments GmbH (Germany)
Univ. Stuttgart (Germany)
Moritz Floess, Univ. Stuttgart (Germany)
Francisco Robles, Georgia Institute of Technology & Emory Univ. School of Medicine (United States)
Stefan Kedenburg, Univ. Stuttgart (Germany)
Harald Giessen, Stuttgart Instruments GmbH (Germany)
Univ. Stuttgart (Germany)


Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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