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Superresolution fluorescence microscopy using saturated modulation quenching (SMoQ)
Author(s): Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Thomas A. Klar; Thomas Berer
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Paper Abstract

In this work, we demonstrate a new technique which has the potential for super-resolution fluorescence imaging. In this technique, similar to STED microscopy, a tightly focused intensity-modulated excitation beam and a donut shaped cw beam are confocally raster-scanned over the sample. In contrast to STED microscopy, both beams need to be absorbed by the fluorophore. A lock-in amplifier is used to measure only the modulated fluorescence. Sufficiently high cw donut beam intensities lead to saturation of the fluorophores in the outer rim of the modulated point spread function which enables resolution enhancement. Theoretically, sub-diffraction resolution can be achieved.

Paper Details

Date Published: 22 February 2019
PDF: 7 pages
Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840U (22 February 2019); doi: 10.1117/12.2510189
Show Author Affiliations
Gregor Langer, Research Ctr. for Non Destructive Testing GmbH (Austria)
Bianca Buchegger, Johannes Kepler Univ. Linz (Austria)
Jaroslaw Jacak, Univ. of Applied Sciences Upper Austria (Austria)
Thomas A. Klar, Johannes Kepler Univ. Linz (Austria)
Thomas Berer, Research Ctr. for Non Destructive Testing GmbH (Austria)

Published in SPIE Proceedings Vol. 10884:
Single Molecule Spectroscopy and Superresolution Imaging XII
Zygmunt Karol Gryczynski; Ingo Gregor; Felix Koberling, Editor(s)

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