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Holographic imaging for tracking and phase retrieval in acoustophoresis platforms
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Paper Abstract

Acoustophoresis devices are proposed as tools for manipulation and diagnostic in microfluidic environments. We demonstrate that their diffusion can be supported and enhanced by Digital Holography. Indeed, this technique covers all the current imaging needs and can stimulate the development of novel applications thanks to its unique features. The numerical refocusing is exploited to control the manipulation during acoustic focusing and acoustic-driven aggregation and to retrieve the 3D trajectories of tracer beads for the ultrasound field calibration. Besides tracking, DH displays its full potential when USs are used to directly manipulate or deform cells. In this case, numerical processing provides information on the sample movement and morphology, with potential applications in the field of diagnostic.

Paper Details

Date Published: 4 March 2019
PDF: 6 pages
Proc. SPIE 10875, Microfluidics, BioMEMS, and Medical Microsystems XVII, 108750F (4 March 2019); doi: 10.1117/12.2510083
Show Author Affiliations
T. Cacace, Institute of Applied Sciences and Intelligent Systems, CNR (Italy)
Univ. Campania L. Vanvitelli (Italy)
P. Memmolo, Institute of Applied Sciences and Intelligent Systems, CNR (Italy)
V. Bianco, Institute of Applied Sciences and Intelligent Systems, CNR (Italy)
M. Paturzo, Institute of Applied Sciences and Intelligent Systems, CNR (Italy)
M. Vassalli, Insittute of Biophysics, National Research Council of Italy (Italy)
M. Fraldi, Univ. of Naples Federico II (Italy)
G. Mensitieri, Univ. of Naples Federico II (Italy)
P. Ferraro, Institute of Applied Sciences and Intelligent Systems, CNR (Italy)


Published in SPIE Proceedings Vol. 10875:
Microfluidics, BioMEMS, and Medical Microsystems XVII
Bonnie L. Gray; Holger Becker, Editor(s)

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