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Thermal microscopy based on quantitative phase imaging for the study of heat-shock proteins at the single cell level (Conference Presentation)
Author(s): Hadrien M. L. Robert; Antoine Federici; Julien Savatier; Stéphanie Vial; Sophia Imperato; Sherazade Aknoun; Hervé Rigneault; Jacob Verghese; Serge Monneret; Julien Polleux; Benoit Wattellier; Guillaume Baffou
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Paper Abstract

In response to heat stress, triggered by a temperature increase of just a few degrees, cells activate a mechanism called the heat-shock response. While conventional global heating processes lead to an overall and slow increase of the temperature, heating processes based on laser illumination enable to achieve fast dynamics on the sub-second time-scale and spatially localized. Thermal imaging using quadriwave lateral shearing interferometry (TIQSI) has been developped by Institut Fresnel in collaboration with PHASICS SA [1]. By quantitatively measuring the transmitted phase this approach is able to measure temperature fields at the microscopic level from thermal-induced refractive index changes of the medium surrounding laser-illuminated plasmonic nanoabsorber. Phase, intensity and temperature images are measured in parallel thanks to a quadriwave lateral shearing interferometry SID4BIO camera, developed by PHASICS. We propose in this article to study the dynamics of the heat-shock response of living cells by using a TIQSI system. A dynamic control heat stress is induced to retinal pigmented epithelial (RPE) cells by illuminating gold nanoparticles used as nanosources of heat [2]. Intensity images of the heat-shock transcription factors (HSF) of cells fluorescently labelled observed in parallel to the heating process reveal the formation of fluorescent granules within the nucleus, a sign of cellular heat-shock response. By heating successively the RPE cells for different laser powers, we measure rise-time and dynamic of the heat-shock for different magnitudes of the stress response. [1] Baffou, G., Bon, P., Savatier, J., Polleux, J., Zhu, M., Merlin, M., ... & Monneret, S. (2012). Thermal imaging of nanostructures by quantitative optical phase analysis. ACS nano, 6(3), 2452-2458. [2] Robert, H.M.L, Savatier, J., Vial, S., Verghese, J., Wattellier, B., … & Baffou, G. (2018) Small, 1801910

Paper Details

Date Published: 4 March 2019
PDF
Proc. SPIE 10887, Quantitative Phase Imaging V, 108870D (4 March 2019); doi: 10.1117/12.2509987
Show Author Affiliations
Hadrien M. L. Robert, Institut Fresnel (France)
Antoine Federici, PHASICS S.A. (France)
Julien Savatier, Institut Fresnel (France)
Stéphanie Vial, Institut Fresnel (France)
Sophia Imperato, PHASICS S.A. (France)
Sherazade Aknoun, PHASICS S.A. (France)
Hervé Rigneault, Institut Fresnel (France)
Jacob Verghese, Max-Planck-Institut für Biochemie (Germany)
Serge Monneret, Institut Fresnel (France)
Julien Polleux, Max-Planck-Institut für Biochemie (Germany)
Ludwig-Maximilians-Univ. München (Germany)
Benoit Wattellier, PHASICS S.A. (France)
Guillaume Baffou, Institut Fresnel (France)


Published in SPIE Proceedings Vol. 10887:
Quantitative Phase Imaging V
Gabriel Popescu; YongKeun Park, Editor(s)

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