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Biocongugation of gold nanoparticles for surface plasmon resonance sensor
Author(s): Sello Manoto; Rudzani Malabi; Saturnin Ombinda-Lemboumba; Patience Mthunzi-Kufa
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Paper Abstract

Over the past few years gold nanoparticle (AuNPs) have become extremely interesting because they possess enhanced optical, electrical and chemical properties. AuNPs have the ability to form robust conjugates with biomolecules such as antibodies and can enhance optical signals making them suitable for a variety of diagnostic applications including the surface plasmon resonance (SPR) technique. SPR is a highly sensitive and label free optical technique which is widely used for detecting biological analytes and analysing the interaction between different types of biomolecules. In this study, bioconjugation was achieved by covalently attaching antibodies to AuNPs and gold coated slides were used as SPR sensor chips in Kretschmann configuration. Several UV/VIS excitation spectra were collected before and after AuNPs were conjugated to antibodies. The results showed that the sensitivity of the SPR system significantly increased because of the bioconjugation of antibodies to AuNPs and this is a promising approach for biosensing applications.

Paper Details

Date Published: 7 March 2019
PDF: 8 pages
Proc. SPIE 10894, Plasmonics in Biology and Medicine XVI, 108941D (7 March 2019); doi: 10.1117/12.2509952
Show Author Affiliations
Sello Manoto, CSIR National Laser Ctr. (South Africa)
Rudzani Malabi, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)
Saturnin Ombinda-Lemboumba, CSIR National Laser Ctr. (South Africa)
Patience Mthunzi-Kufa, CSIR National Laser Ctr. (South Africa)
Univ. of South Africa (South Africa)

Published in SPIE Proceedings Vol. 10894:
Plasmonics in Biology and Medicine XVI
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)

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