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Polarization wavefront shaping for quantitative phase contrast imaging by axially-offset differential interference contrast (ADIC) microscopy
Author(s): Changqin Ding; Chen Li; Fengyuan Deng; Garth J. Simpson
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Paper Abstract

Axially-offset differential interference contrast (ADIC) microscopy was developed for quantitative phase contrast imaging (QPI) by using polarization wavefront shaping approach with a matched pair of micro-retarder arrays. In ADIC microscopy, wavefront shaping with a micro-retarder array (μRA) produces a pattern of half-wave retardance varying spatially in the azimuthal orientation of the fast-axis. For a linearly polarized input beam, the polarization pattern induced from the linearly polarized plane wave through the μRA is identical to the interference between a slightly diverging right circularly polarized (RCP) and a slightly converging left circularly polarized (LCP) plane wave. Using a 10× objective, two axially offset foci separated by 70 μm are consequently generated from the patterned wavefront with orthogonal polarization states, serving as the sample and reference focal planes respectively for QPI. A paired μRA in transmission coherently recombines the two orthogonal components to recover the incident polarization state in the absence of sample. The large spatial offset (roughly 1/10 of the field of view) between the two foci provides a stable and uniform reference. Quantitative phase contrast images are directly recovered from sample-scan measurements with a single-channel detector and lock-in amplification with fast polarization modulation. This method has been successfully used for bio-sample imaging, nanoparticle detection and refractive index calculation of silica microbeads.

Paper Details

Date Published: 4 March 2019
PDF: 8 pages
Proc. SPIE 10887, Quantitative Phase Imaging V, 1088728 (4 March 2019); doi: 10.1117/12.2509929
Show Author Affiliations
Changqin Ding, Purdue Univ. (United States)
Chen Li, Purdue Univ. (United States)
Fengyuan Deng, Purdue Univ. (United States)
Garth J. Simpson, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 10887:
Quantitative Phase Imaging V
Gabriel Popescu; YongKeun Park, Editor(s)

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