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Diatomaceous earth/polydopamine hybrid microstructures as enzymes support for biological applications
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Paper Abstract

Diatom microalgae represent the most abundant source of mesoporous biosilica in our planet. Their fossil derivative, diatomaceous earth (DE), consisting of diverse algal debris with nanostructured morphologies, is envisaged as a low cost silica support for biological applications. Intriguing features such as high surface/volume ratio and biocompatibility as well as unique absorption and confinement properties, make DE a suitable mesoporous support for biomolecules’ immobilization and stabilization. In this work, the model enzyme laccase was immobilized on DE using a polydopamine (PDA) coating that entraps a layer of protein molecules weakly interacting with DE. The DE/PDA/Laccase material, produced in aqueous solution under mild environment-friendly conditions, was characterized by spectroscopy and microscopy. The kinetic parameters and the recycle of the laccase were evaluated. This new hybrid material is in principle suitable for biomedical applications and for bioremediation in different environments.

Paper Details

Date Published: 7 March 2019
PDF: 10 pages
Proc. SPIE 10892, Colloidal Nanoparticles for Biomedical Applications XIV, 108920E (7 March 2019); doi: 10.1117/12.2509879
Show Author Affiliations
Danilo Vona, Univ. degli Studi di Bari Aldo Moro (Italy)
Nicoletta Mezzina, Univ. degli Studi di Bari Aldo Moro (Italy)
Stefania R. Cicco, Istituto di Chimica dei Composti OrganoMetallici, CNR (Italy)
Gabriella Leone, Univ. degli Studi di Bari Aldo Moro (Italy)
Italian Institute of Technology (Italy)
Roberta Ragni, Univ. degli Studi di Bari Aldo Moro (Italy)
Marco Lo Presti, Univ. degli Studi di Bari Aldo Moro (Italy)
Gianluca M. Farinola, Univ. degli Studi di Bari Aldo Moro (Italy)


Published in SPIE Proceedings Vol. 10892:
Colloidal Nanoparticles for Biomedical Applications XIV
Marek Osiński; Wolfgang J. Parak, Editor(s)

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