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Raman imaging of α-synuclein aggregates in a rat model of Parkinson’s disease
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Paper Abstract

Aggregates of misfolded α-Synuclein in the brain represent a hallmark of Parkinson’s disease (PD). In patients and animal models, phosphorylated α-Synuclein was detected in the gut, hence, raising the hypothesis that early-stage PD could be diagnosed based on colon tissues. Marker-independent technologies represent an ideal method to monitor disease progression and potentially detect early-stage aggregated α-Synuclein in vivo. Here, formalin-fixed, paraffinembedded colon tissues of a transgenic rat model were analyzed using Raman imaging. Detailed spectral and imagebased analysis was performed indicating the major spectral shifts that alter in PD rat tissues in the amide I region. Peak fitting and multivariate analysis specified an increase of β-sheet proteins in transgenic rat colon compared with wild-type colon. In summary, Raman imaging is capable to detect α-Synuclein aggregates in colon tissues of a PD rat model, indicating that it could be a useful tool to support diagnosis in PD pathology.

Paper Details

Date Published: 4 March 2019
PDF: 7 pages
Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 1088116 (4 March 2019); doi: 10.1117/12.2509751
Show Author Affiliations
Eva Brauchle, Eberhard Karls Univ. (Germany)
Natural and Medical Sciences Institute at the Univ. of Tübingen (Germany)
Fide Sevgi, Eberhard Karls Univ. (Germany)
Daniel Carvajal Berrio, Eberhard Karls Univ. (Germany)
Katja Schenke-Layland, Eberhard Karls Univ. (Germany)
Natural and Medical Sciences Institute at the Univ. of Tübingen (Germany)
David Geffen School of Medicine at UCLA (United States)
Nicolas Casadei, Eberhard Karls Univ. (Germany)
Olaf Riess, Eberhard Karls Univ. (Germany)
Madhuri S. Salker, Eberhard Karls Univ. (Germany)
Yogesh Singh, Eberhard Karls Univ. (Germany)


Published in SPIE Proceedings Vol. 10881:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII
Daniel L. Farkas; Attila Tarnok; James F. Leary, Editor(s)

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