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Vibrational imaging of metabolic dynamics in animals by DO-SRS (Conference Presentation)
Author(s): Wei Min; Lingyan Shi
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Paper Abstract

Metabolic dynamics is essential to unraveling the mechanistic basis of many biological processes, and cannot be imaged in vivo by using traditional methods. Here we developed a new method that combines D2O probing and SRS (DO-SRS) to visualize metabolic dynamics in live animals. The enzymatic incorporation of D2O-derived deuterium (D) into biomolecules will generate carbon-deuterium (C-D) bonds in macromolecules. We developed spectral unmixing methods to obtain C-D signals with macromolecular selectivity, and applied DO-SRS to reveal metabolic dynamics in C. elegans, zebrafish, and mice, to study developmental biology, aging, and tissue homeostasis, and to visualize tumor boundaries and metabolic heterogeneity.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821T (4 March 2019); doi: 10.1117/12.2509742
Show Author Affiliations
Wei Min, Columbia Univ. (United States)
Lingyan Shi, Columbia Univ. (United States)

Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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