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Rapid volumetric imaging with light-sheet microscopy
Author(s): Reto Fiolka
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Paper Abstract

Light-sheet fluorescence microscopy (LSFM) enables both sensitive and rapid volumetric imaging, from single cells to multicellular organisms. Nevertheless, LSFM faces technological and physical limitations on the volumetric acquisition speed. We have realized that there are certain geometrical arrangements that allow lossless parallelization of the LSFM acquisition process. Thereby the volumetric acquisition rate can be increased without inducing higher amounts of phototoxicity or requiring faster detector technologies. We present two implementations of parallelized light-sheet microscopy and discuss applications that either benefit from the increased acquisition speed or improved sensitivity of these systems.

Paper Details

Date Published: 4 March 2019
PDF: 8 pages
Proc. SPIE 10889, High-Speed Biomedical Imaging and Spectroscopy IV, 108890F (4 March 2019); doi: 10.1117/12.2509726
Show Author Affiliations
Reto Fiolka, The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States)


Published in SPIE Proceedings Vol. 10889:
High-Speed Biomedical Imaging and Spectroscopy IV
Kevin K. Tsia; Keisuke Goda, Editor(s)

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