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Feasibility study of limited-angle reconstruction based in vivo optical projection tomography
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Paper Abstract

Optical projection tomography(OPT) provides an approach to recreating three-dimensional images of small biological specimens. Light traverses through a straight line to achieve a homogeneous illumination of the specimen. As the specimens in the conventional OPT could not survive or the survival time was too short, this paper proposes a new type of sample fixation method for OPT imaging. The specimen was anaesthetized in a petri dish, and the dish was fixed under the rotational stage of our homemade OPT system for imaging. This method can reduce the damage to the specimen and be more conducive to the continuous observation for in vivo OPT. However, the sample fixation causes the problem of insufficient sampling. To obtain optical projection tomographic image with insufficient samples, this paper uses the iterative reconstruction algorithm combining with the prior information to solve the inverse reconstruction problem.

Paper Details

Date Published: 21 February 2019
PDF: 9 pages
Proc. SPIE 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, 108830S (21 February 2019); doi: 10.1117/12.2509485
Show Author Affiliations
Nan Wang, Xidian Univ. (China)
Duofang Chen, Xidian Univ. (China)
Cuiping Bao, Xidian Univ. (China)
Jimin Liang, Xidian Univ. (China)
Xueli Chen, Xidian Univ. (China)
Shouping Zhu, Xidian Univ. (China)


Published in SPIE Proceedings Vol. 10883:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI
Thomas G. Brown; Tony Wilson, Editor(s)

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