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Towards laser-assisted microfluidic-cell transfection
Author(s): Hans Georg Breunig; Ana Batista; Aisada König; Karsten König
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Paper Abstract

By short-term irradiation of a cell with focused laser pulses, the cell membrane can be made transiently permeable. This "optoporation" allows for foreign macro molecules to pass this otherwise impenetrable barrier and enter the cell interior enabling a highly efficient and “hands-off” laser-assisted transfection of cells. We intend to combine a precise flow control of cells with the laser-assisted-transfection technique by employing microfluidics to control the cell flow through the laser-cell interaction region. The precise flow control will allow for a high throughput of cells and combine microfluid cell control with the advantages of the highly efficient laser-assisted transfection scheme. The development of the setup is still in progress. Here, the background and key features are outlined.

Paper Details

Date Published: 4 March 2019
PDF: 4 pages
Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108811W (4 March 2019); doi: 10.1117/12.2509442
Show Author Affiliations
Hans Georg Breunig, Univ. des Saarlandes (Germany)
Ana Batista, Univ. des Saarlandes (Germany)
Aisada König, Univ. des Saarlandes (Germany)
Karsten König, Univ. des Saarlandes (Germany)

Published in SPIE Proceedings Vol. 10881:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII
Daniel L. Farkas; Attila Tárnok; James F. Leary, Editor(s)

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