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Phasor plot analysis using low pass filter for high-speed FLIM
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Paper Abstract

Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.

Paper Details

Date Published: 22 February 2019
PDF: 4 pages
Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840P (22 February 2019); doi: 10.1117/12.2509325
Show Author Affiliations
Dongeun Kim, Yonsei Univ. (Korea, Republic of)
Wonsang Hwang, Yonsei Univ. (Korea, Republic of)
Youngjae Won, Osong Medical Innovation Foundation (Korea, Republic of)
Sucbei Moon, Kookmin Univ. (Korea, Republic of)
Sang Yoon Lee, INTEKPLUS Co., Ltd. (Korea, Republic of)
Mingu Kang, INTEKPLUS Co., Ltd. (Korea, Republic of)
Won Sub Han, INTEKPLUS Co., Ltd. (Korea, Republic of)
Dug Young Kim, Yonsei Univ. (Korea, Republic of)


Published in SPIE Proceedings Vol. 10884:
Single Molecule Spectroscopy and Superresolution Imaging XII
Zygmunt Karol Gryczynski; Ingo Gregor; Felix Koberling, Editor(s)

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