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Optical fiber based light scattering detection in microfluidic droplets
Author(s): Shulin Wohlfeil; Sundar Hengoju; Anne-Sophie Munser; Miguel Tovar; Oksana Shvydkiv; Martin Roth; Sven Schröder; Erik Beckert; Ramona Eberhardt; Andreas Tünnermann
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Paper Abstract

Droplet based microfluidic technology is a miniaturized platform for microbial analysis on picoliter scale. With its costefficiency, high-throughput and feasibility of complex handling protocols, droplet microfluidics is a favorable platform for applications such as microorganism screening or synthetic biology. Scattered-light-based microbial detection, in comparison to the widely used fluorescent-label-based approach, provides a contact-free and label-free, yet sensitive measuring solution. The angular dependency of scattered light delivers an elaborate information about the morphology and the physical properties, e.g. size and refractive index, of microbial samples. Due to the complexity and ambiguity of the droplet contents, an angle resolved scattered light detection system could provide powerful method for a label-free identification and quantification of the microbes in droplets. In this paper, a novel approach of light scattering measurement in Polydimethylsiloxane (PDMS) microfluidic chips is presented, engaging optical fibers for a light-scattering-based on-chip microbial detection. Optical fibers, with their fast readout and compact size, are very suitable for easier system integration towards flexible and versatile lab-on-a-chip applications.

Paper Details

Date Published: 4 March 2019
PDF: 7 pages
Proc. SPIE 10875, Microfluidics, BioMEMS, and Medical Microsystems XVII, 1087516 (4 March 2019); doi: 10.1117/12.2509248
Show Author Affiliations
Shulin Wohlfeil, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Sundar Hengoju, Hans-Knöll-Institute (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Anne-Sophie Munser, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
Miguel Tovar, Hans Knöll Institute (Germany)
Oksana Shvydkiv, Hans-Knöll-Institute (Germany)
Martin Roth, Hans Knöll Institute (Germany)
Sven Schröder, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Erik Beckert, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Ramona Eberhardt, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Andreas Tünnermann, Fraunhofer-Institut für Angewandte Optik und Feinmechanik (Germany)
Friedrich-Schiller-Univ. Jena (Germany)


Published in SPIE Proceedings Vol. 10875:
Microfluidics, BioMEMS, and Medical Microsystems XVII
Bonnie L. Gray; Holger Becker, Editor(s)

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