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Development of microscopic adaptive optics using image correlation
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Paper Abstract

Adaptive optics (AO) is a promising technique for correcting wavefront errors induced by complex structures of biological samples which significantly causes image degradation. We develop a microscopic AO system with a Shack-Hartmann wavefront sensor based on image correlation. The correlation-based wavefront sensing is feasible using an extended object under bright-field illumination, as well as spot fluorescence. To make the correlation-based sensing more reliable, we newly introduce a technique of excluding sub-images with insufficient quality. We show experimental results under a variety of conditions for objects, light sources, and wavefront error sources. In any cases, we confirmed that the AO system effectively worked so as to improve image qualities.

Paper Details

Date Published: 20 February 2019
PDF: 6 pages
Proc. SPIE 10886, Adaptive Optics and Wavefront Control for Biological Systems V, 1088617 (20 February 2019); doi: 10.1117/12.2509165
Show Author Affiliations
Yusuke Honma, Kitami Institute of Technology (Japan)
Noriaki Miura, Kitami Institute of Technology (Japan)
Hayao Kikuchi, Kitami Institute of Technology (Japan)
Masayuki Hattori, National Astronomical Observatory of Japan (Japan)
Yosuke Tamada, National Institute for Basic Biology (Japan)
Atsushi Matsuda, National Institute of Information and Communications Technology (Japan)

Published in SPIE Proceedings Vol. 10886:
Adaptive Optics and Wavefront Control for Biological Systems V
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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