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A chemist’s view of inflammation in contusion injured spinal cord in a rat model: noninvasive, noncontact, in vivo Raman spectroscopy minutes to hours after injury
Author(s): Seth Fillioe; Kyle Kelly Bishop; Sai Han Tun; Paul Dent; Bin Deng; Charles M. Peterson; Jerry Goodisman; Julie Hasenwinkel; J. Chaiken
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Paper Abstract

The initial biological response to spinal cord injury is initiated by intra- and extracellular chemical signals. We compare Raman spectra of injured spinal cord obtained minutes after injury to those of uninjured spinal cord to obtain chemical information that precedes the biological response. We studied 29 rats including both Injured and Control using Raman spectra of spinal cords in vivo. Principal Component Analysis (PCA) indicates that <99% of the variation of these spectra across both Injured and Control groups is accounted for with 3 components. The first component does not vary significantly representing structural materials. The second and third components reflect the variation in the chemistry of the cerebrospinal fluid. We demonstrate the first noninvasive in vivo measurement of pH in the CSF using only Raman spectra. We hypothesize that the earliest inflammatory response to mild contusive injury reflects the chemistry of inorganic phosphate present at abnormally high concentrations, likely due to physical disruption of the blood-brain barrier in the choroid plexus and/or mitochondrial release of phosphate, reacting with CSF water.

Paper Details

Date Published: 7 March 2019
PDF: 15 pages
Proc. SPIE 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 108630G (7 March 2019); doi: 10.1117/12.2509082
Show Author Affiliations
Seth Fillioe, Syracuse Univ. (United States)
Kyle Kelly Bishop, Syracuse Univ. (United States)
Sai Han Tun, Syracuse Univ. (United States)
Paul Dent, Syracuse Univ. (United States)
Bin Deng, Syracuse Univ. (United States)
Charles M. Peterson, Syracuse Univ. (United States)
Jerry Goodisman, Syracuse Univ. (United States)
Julie Hasenwinkel, Syracuse Univ. (United States)
J. Chaiken, Syracuse Univ. (United States)

Published in SPIE Proceedings Vol. 10863:
Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II
Tianhong Dai; Jürgen Popp; Mei X. Wu, Editor(s)

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