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A diffuse optical tomography system for whole-brain functional imaging in mice using multiple camera views (Conference Presentation)
Author(s): Zachary E. Markow; Matthew D. Reisman; Adam Q. Bauer; Adam T. Eggebrecht; Mark A. Anastasio; Joseph P. Culver
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Paper Abstract

For functional neuroimaging, existing small-animal diffuse optical tomography (DOT) systems either do not provide adequate temporal sampling rates, have sparse spatial sampling, or have limited three-dimensional fields of view. To achieve adequate frame rates (1-10 Hz), we have constructed a system using sCMOS detection-based DOT, with asymmetric measurements, with many (>10,000) detectors and fewer (<100) structured illumination patterns (using digital micromirror devices: DMDs). The system employs multiple views, involving multiple cameras and illuminators, to provide a three-dimensional field of view. To coregister the measurements with the mouse head anatomy, we developed a surface profiling method in which point illumination patterns are scanned over the mouse head and combined with calibration data to create three-dimensional point clouds and meshes representing the head. We applied this method to a 3D-printed figurine, and the resulting mesh had surface vertices whose positions deviated 0.4 ± 0.2 mm (mean ± SD) from the original "ground truth" mesh that had been employed to 3D-print the figurine. To evaluate the imaging system's resolution, field of view, and sensitivity versus depth, we placed simulated activations at different depths within a tissue model of a real mouse head imaged with our surface profiling method. Results indicate that this imaging system is sensitive to absorption changes at depths of >3 mm. In addition, a partial (one-camera, one-illuminator) version of the system successfully imaged neural activations evoked by forepaw stimulation of a live mouse.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10874, Optical Tomography and Spectroscopy of Tissue XIII, 108741G (4 March 2019); doi: 10.1117/12.2508997
Show Author Affiliations
Zachary E. Markow, Washington Univ. in St. Louis (United States)
Matthew D. Reisman, Washington Univ. in St. Louis (United States)
Adam Q. Bauer, Washington Univ. in St Louis (United States)
Adam T. Eggebrecht, Washington Univ. in St Louis (United States)
Mark A. Anastasio, Washington Univ. in St. Louis (United States)
Joseph P. Culver, Washington Univ. in St Louis (United States)

Published in SPIE Proceedings Vol. 10874:
Optical Tomography and Spectroscopy of Tissue XIII
Sergio Fantini; Bruce J. Tromberg; Eva Marie Sevick-Muraca; Paola Taroni, Editor(s)

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