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Refractive index properties of the retina accessed by multi-wavelength digital holographic microscopy
Author(s): Álvaro Barroso Peña; Steffi Ketelhut; Peter Heiduschka; Geburg Nettels-Hackert; Jürgen Schnekenburger; Björn Kemper
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Paper Abstract

The refractive index (RI) of the retina and its dispersion are essential parameters in ophthalmologic imaging. However, the spatial RI distribution in retinal tissue is difficult to access. We explored the capabilities of multispectral quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for label-free refractive index characterization of dissected murine retina. The retrieved tissue refractive indices are in agreement with previously reported values for living cells and dissected tissues. Moreover, the detected spatial refractive index distributions correlate with results from complementary conducted OCT investigations. In summary, multispectral DHM is a promising tool for label-free characterization of optical retina properties.

Paper Details

Date Published: 21 February 2019
PDF: 6 pages
Proc. SPIE 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, 108830X (21 February 2019); doi: 10.1117/12.2508976
Show Author Affiliations
Álvaro Barroso Peña, Univ. of Münster (Germany)
Steffi Ketelhut, Univ. of Münster (Germany)
Peter Heiduschka, Univ. of Muenster Medical Ctr. (Germany)
Geburg Nettels-Hackert, Univ. of Muenster Medical Ctr. (Germany)
Jürgen Schnekenburger, Univ. of Münster (Germany)
Björn Kemper, Univ. of Münster (Germany)


Published in SPIE Proceedings Vol. 10883:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI
Thomas G. Brown; Tony Wilson, Editor(s)

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