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Development of a high-resolution high-sensitivity compressive Raman microscope (Conference Presentation)
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Paper Abstract

The high data bandwidth of Raman imaging precludes high-speed spectroscopic imaging. Conversely, emerging compressive sensing hyperspectroscopy techniques could, in principle, address this issue by using undersampling methodologies with computational reconstructions. However, compressive spectrometer layouts have prohibitive losses for low-light levels applications, such as in the spontaneous Raman imaging of dynamic biological specimens. These losses are due to the fact that high-sensitivity light detectors (photo-counters) have too small active area (typically 100 um) compared to the size of digital micromirror devices (DMD) (~10 mm) used in most compressive layouts. Inspired by pulse shaping techniques of ultrafast spectroscopy, we present a new programmable spectrometer layout with high-throughput and large spectral coupling bandwidths. Exploiting amplitude spectral modulation with DMD allows conventional and compressive Raman imaging and spectroscopy acquisitions with shot-noise-limited sensitivity. With this spectrometer, we demonstrate compressed hyperspectroscopy at faster speeds and at lower costs than traditional cameras used in Raman imaging applications. We showcase imaging of biological specimens at high spatial resolution (250 nm).

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10932, Emerging Digital Micromirror Device Based Systems and Applications XI, 1093205 (4 March 2019); doi: 10.1117/12.2508927
Show Author Affiliations
Benneth Sturm, Lab. Pierre Aigrain (France)
Fernando Soldevila, Univ. Jaume I (Spain)
Enrique Tajahuerce, Univ. Jaume I (Spain)
Sylvain Gigan, Lab. Kastler Brossel (France)
Hilton B. de Aguiar, Lab. Pierre Aigrain (France)

Published in SPIE Proceedings Vol. 10932:
Emerging Digital Micromirror Device Based Systems and Applications XI
Michael R. Douglass; John Ehmke; Benjamin L. Lee, Editor(s)

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