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Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: the case of nanostructured porous silicon interferometers
Author(s): S. Mariani; V. Robbiano; L. M. Strambini; A. Debrassi; G. Egri; L. Dähne; G. Barillaro
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Paper Abstract

The nano-assembly of charged polyelectrolytes via layer-by-layer (LbL) technology on porous silicon (PSi) interferometers is here demonstrated as an effective biofunctionalization approach for high-sensitivity/selectivity labelfree optical biosensing, using streptavidin/biotin affinity detection as case study. Nanostructured PSi interferometers are biofunctionalized with a nano-assembly of a positively-charged polyelectrolyte, namely, PAH (poly(allylamine hydrochloride)), and a negatively-charged biotinylated polyelectrolyte, namely, b-PMAA (poly(methacrylic acid)), via LbL technology. The nano-assembly is stable under operating conditions and enables the selective and sensitive detection of streptavidin with a sub-picomolar detection limit (namely, DL=0.6 pM), which is 105-fold lower than that achieved with PSi interferometers biofunctionalized using standard silane chemistry. Remarkably, the analytical performance achieved for LbL-biofunctionalized PSi interferometers is comparable to those of state-of-the-art label-free photonic and plasmonic platforms.

Paper Details

Date Published: 7 March 2019
PDF: 7 pages
Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 1089503 (7 March 2019); doi: 10.1117/12.2508847
Show Author Affiliations
S. Mariani, Univ. di Pisa (Italy)
Dipartimento di Ingegneria dell’Informazione (Italy)
V. Robbiano, Univ. di Pisa (Italy)
Dipartimento di Ingegneria dell’Informazione (Italy)
L. M. Strambini, Consiglio Nazionale delle Ricerche (Italy)
A. Debrassi, Surflay Nanotec GmbH (Germany)
G. Egri, Surflay Nanotec GmbH (Germany)
L. Dähne, Surflay Nanotec GmbH (Germany)
G. Barillaro, Univ. di Pisa (Italy)
Consiglio Nazionale delle Ricerche (Italy)


Published in SPIE Proceedings Vol. 10895:
Frontiers in Biological Detection: From Nanosensors to Systems XI
Amos Danielli; Benjamin L. Miller; Sharon M. Weiss, Editor(s)

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