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Optogenetic control of cytokinesis shows that polarized trafficking governs the process
Author(s): Jean A. Castillo-Badillo; Xenia Meshik; Suyash Harlalka; N. Gautam
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Paper Abstract

Optogenetics allows for the study of signaling pathways during a variety of cellular processes. Here we use subcellular optogenetics to study the role of vesicular trafficking in cytokinesis. Optogenetically activating RhoA in the cell midline induces cytokinesis and intercellular bridge formation. RhoA activates the actomyosin contractility network, leading to retrograde plasma membrane flow, localized decrease in membrane tension, and increase in endocytosis at the cell middle. Endocytic vesicles and trafficking proteins localize to the intercellular bridge. Perturbation of these proteins inhibits furrow formation. Thus polarized endocytosis and exocytosis in the first steps of cytokinesis provide new membrane to the middle of the cell and allow the cell to build the cleavage furrow and intercellular bridge.

Paper Details

Date Published: 22 February 2019
PDF: 7 pages
Proc. SPIE 10866, Optogenetics and Optical Manipulation 2019, 1086609 (22 February 2019); doi: 10.1117/12.2508792
Show Author Affiliations
Jean A. Castillo-Badillo, Washington Univ. in St Louis (United States)
Xenia Meshik, Washington Univ. in St Louis (United States)
Suyash Harlalka, Washington Univ. in St Louis (United States)
N. Gautam, Washington Univ. in St. Louis (United States)

Published in SPIE Proceedings Vol. 10866:
Optogenetics and Optical Manipulation 2019
Samarendra K. Mohanty; E. Duco Jansen, Editor(s)

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