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Imaging mitochondrial matrix viscosity in live cells via fluorescence lifetime imaging (FLIM) of fluorescent molecular rotors
Author(s): I. Emilie Steinmark; Arjuna L. James; Cécile A. Dreiss; Gokhan Yahioglu; Klaus Suhling
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Paper Abstract

Here we use the combination of fluorescent molecular rotors (FMR) and fluorescence lifetime imaging (FLIM) to image matrix viscosity in live cells. We use non-cancerous, epithelial human cornea (HCE) cells as a model system. We find that mitochondrial matrix viscosity varies between individual cells and even between individual organelles, showcasing the potential of viscosity imaging for cell biology purposes.

Paper Details

Date Published: 7 March 2019
PDF: 7 pages
Proc. SPIE 10893, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI, 108930K (7 March 2019); doi: 10.1117/12.2508676
Show Author Affiliations
I. Emilie Steinmark, King's College London (United Kingdom)
Arjuna L. James, King's College London (United Kingdom)
Cécile A. Dreiss, King's College London (United Kingdom)
Gokhan Yahioglu, Imperial College London (United Kingdom)
Klaus Suhling, King's College London (United Kingdom)


Published in SPIE Proceedings Vol. 10893:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XI
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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