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Controlled membrane depolarization through photothermal effects of tethered gold nanoparticles
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Paper Abstract

Nanoparticle (NP) bioconjugates have an important role in the development of photothermal (PT) therapy, a promising noninvasive approach wherein the NP acts as a light harvesting antenna to convert light into thermal energy to control cellular function. NP-mediated PT control of cellular membrane potential has gained significant interest in recent years as membrane potential regulates proliferation, migration, action potentials (in neurons), and contraction (in muscle cells). Recently gold nanoparticles (AuNPs) and Au nanorods have been demonstrated to induce action potentials via light-induced thermal activation of membrane tethered NPs. Spherical AuNPs have an efficient plasmonic output and are easily modified to interface with the cell surface. We demonstrate here that 20 nm diameter spherical AuNPs (tethered to the plasma membrane by a cholesterol moiety) transduce incident 532 nm light into proximal membrane heating that induces depolarization of membrane potential. Using these NP bioconjugates, we show the ability to controllably induce action potentials in dorsal root ganglion neurons and to control the membrane potential of rat pheochromocytoma cells. The ability to use light-actuated NP conjugates to control cellular behavior is an emerging research field with implications for neuronal and muscle cell modulation as well as in cancer therapeutics.

Paper Details

Date Published: 7 March 2019
PDF: 6 pages
Proc. SPIE 10892, Colloidal Nanoparticles for Biomedical Applications XIV, 108920M (7 March 2019); doi: 10.1117/12.2508662
Show Author Affiliations
Megan E. Muroski, U.S. Naval Research Lab. (United States)
Okhil Nag, U.S. Naval Research Lab. (United States)
Eunkeu Oh, U.S. Naval Research Lab. (United States)
KeyW Corp. (United States)
Alan L. Huston, U.S. Naval Research Lab. (United States)
James B. Delehanty, U.S. Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 10892:
Colloidal Nanoparticles for Biomedical Applications XIV
Marek Osiński; Wolfgang J. Parak, Editor(s)

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