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Cardiac recovery after pacing as an indicator of cardiac health in Drosophila (Conference Presentation)
Author(s): Jing Men; Airong Li; Jason Jerwick; Jixu Yu; Rudolph Tanzi; Chao Zhou
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Paper Abstract

The ability to quickly recover to a resting heart rate after physical exertion is an indicator of heart health. Experiments with humans and other mammals use exercise to induce physiological distress and to increase the heart rate in subjects. In Drosophila models, it is not feasible to induce increased physical activity while simultaneously recording their heartrate. Cardiac pacing can be used as an analog to physical activity because it forces the fly heart to follow a pacing rate which induces physiological distress. In the established Drosophila models, we used non-invasive red light optogenetic pacing to activate or inhibit physical activities while simultaneously record the heart rate (HR) using the optical coherence microscopy technique. In ReaChR flies, a recovery period with a gradually increasing HR was observed after inducing tachycardia through the red light stimulation. The maximum HR and the time period before reaching the resting heart rate after pacing ceases were studied in the fruit flies. Physiological distress was also induced by reducing or halting the NpHR Drosophila’s heart rate through red light stimuli. After induced bradycardia pacing and cardiac arrest a recovery period of rapid heart beating was observed. The cardiac recovery after pacing could be extensively used as an indicator in understanding the correlation of age with cardiac deterioration in different animal models and humans.

Paper Details

Date Published: 7 March 2019
Proc. SPIE 10866, Optogenetics and Optical Manipulation 2019, 108660R (7 March 2019); doi: 10.1117/12.2508519
Show Author Affiliations
Jing Men, Lehigh Univ. (United States)
Airong Li, Massachusetts General Hospital, Harvard Medical School (United States)
Jason Jerwick, Lehigh Univ. (United States)
Jixu Yu, Massachusetts General Hospital, Harvard Medical School (United States)
Rudolph Tanzi, Massachusetts General Hospital, Harvard Medical School (United States)
Chao Zhou, Lehigh Univ. (United States)

Published in SPIE Proceedings Vol. 10866:
Optogenetics and Optical Manipulation 2019
Samarendra K. Mohanty; E. Duco Jansen, Editor(s)

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