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Analysis of microorganisms, chlorinated hydrocarbons and hyaluronic acid gel using Raman based optofluidic techniques and SERS
Author(s): Ota Samek; Zdenek Pilat; Silvie Bernatova; Jan Jezek; Tereza Klementova; Martin Kizovsky; Pavel Zemanek; Katarina Rebrosova; Filip Ruzicka
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Paper Abstract

We report on the development of a set of Raman based techniques to monitor a large variety of biological and chemical analytes, such as various microorganisms, gels of hyaluronic acid and selected halogenated hydrocarbons using Raman spectroscopy, Raman tweezers and surface-enhanced Raman spectroscopy (SERS). We analyzed individual microbial cells with Raman tweezers to provide solutions for fast and label-free identification of specific bacterial or yeast species. We designed an optofluidic SERS platform for quantification of sub-millimolar concentrations of halogenated environmental pollutants such as 1,2,3-trichloropropane and chloroform. We also examined the gel structure of hyaluronic acid by Raman spectroscopy.

Paper Details

Date Published: 7 March 2019
PDF: 8 pages
Proc. SPIE 10894, Plasmonics in Biology and Medicine XVI, 108940D (7 March 2019); doi: 10.1117/12.2508425
Show Author Affiliations
Ota Samek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Zdenek Pilat, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Silvie Bernatova, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Jan Jezek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Tereza Klementova, Brno Univ. of Technology (Czech Republic)
Martin Kizovsky, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments of the CAS, v.v.i. (Czech Republic)
Katarina Rebrosova, St. Anne's Univ. Hospital Brno (Czech Republic)
Filip Ruzicka, St. Anne's Univ. Hospital Brno (Czech Republic)


Published in SPIE Proceedings Vol. 10894:
Plasmonics in Biology and Medicine XVI
Tuan Vo-Dinh; Ho-Pui A. Ho; Krishanu Ray, Editor(s)

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