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Confocal Raman microscopy and non linear microscopy to study human enamel incipient lesions
Author(s): Rand Al-Obaidi; Hervé Tassery; Thierry Cloitre; Csilla Gergely; Frédéric Cuisinier; Hamideh Salehi
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Paper Abstract

Incipient enamel lesions represent an early phase of dental caries. They can be produced in-vitro by pH cycling. Raman spectral maps showed that the higher the organic/mineral ratio in the demineralized enamel, the lower is the intensity of mineral content in the same zone. Moreover, the evident red shift of the two-photon excited fluorescence (2PEF) spectrum from carious enamel, compared to that from sound enamel is typical of carious lesion formation. Second harmonic generation (SHG) signal was also observed in the lesion zone. The obtained results demonstrate a great potential for Raman, 2PEF and SHG photonic signals in diagnostic of incipient carious lesions.

Paper Details

Date Published: 28 February 2019
PDF: 5 pages
Proc. SPIE 10857, Lasers in Dentistry XXV, 108570C (28 February 2019); doi: 10.1117/12.2508306
Show Author Affiliations
Rand Al-Obaidi, Univ. Montpellier (France)
Hervé Tassery, Univ. Montpellier (France)
Thierry Cloitre, Univ. Montpellier (France)
Csilla Gergely, Univ. Montpellier (France)
Frédéric Cuisinier, Univ. Montpellier (France)
Hamideh Salehi, Univ. Montpellier (France)

Published in SPIE Proceedings Vol. 10857:
Lasers in Dentistry XXV
Peter Rechmann; Daniel Fried, Editor(s)

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