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Broadband hyperspectral stimulated Raman scattering microscopy with a parabolic fiber amplifier source
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Paper Abstract

Hyperspectral Stimulated Raman scattering (hsSRS) microscopy has recently emerged as a powerful non-destructive technique for label-free chemical imaging of biological samples. In most hsSRS imaging experiments, the SRS spectral range is limited by the total bandwidth of the excitation laser to ~300 cm-1 and spectral resolution of ~25 cm-1. Here we present a novel approach for broadband hsSRS microscopy based on parabolic fiber amplification to provide linearly chirped broadened Stokes pulses. This novel hsSRS instrument provides >600 cm-1 spectral coverage and ~10 cm-1 spectral resolution. We further demonstrated broadband hsSRS imaging of the entire Raman fingerprint region for resolving distribution of major biomolecules in fixed cells. Moreover, we applied broadband hsSRS in imaging amyloid plaques in human brain tissue with Alzheimer’s disease.

Paper Details

Date Published: 4 March 2019
PDF: 13 pages
Proc. SPIE 10889, High-Speed Biomedical Imaging and Spectroscopy IV, 1088910 (4 March 2019); doi: 10.1117/12.2508104
Show Author Affiliations
Benjamin Figueroa, Univ. of Washington (United States)
Walter Fu, Cornell Univ. (United States)
Tai Nguyen, Univ. of Washington (United States)
The Univ. of Southern California (United States)
Kseniya Shin, Univ. of Washington (United States)
Bryce Manifold, Univ. of Washington (United States)
Frank Wise, Cornell Univ. (United States)
Dan Fu, Univ. of Washington (United States)

Published in SPIE Proceedings Vol. 10889:
High-Speed Biomedical Imaging and Spectroscopy IV
Kevin K. Tsia; Keisuke Goda, Editor(s)

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