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Cell membrane molecular dynamics under a NIR focused laser
Author(s): Remy Avila; Elisa Tamariz; Norma Medina-Villalobos; Jordi Andilla; Maria Marsal; Pablo Loza-Alvarez
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Paper Abstract

Light interaction with live cells can lead to important effects. Near infrared (NIR) lasers are used in therapies to diminish inflammation and pain. In a microscopic scale, NIR laser light has been used to stimulate and guide the growth of cells like neurons and fibroblasts. However, it is still unclear what NIR laser radiation provokes to the cells at the molecular and biophysical levels. In this contribution we report the effects of a continuous wave 810-nm laser on the plasma membrane of in vivo 3T3 fibroblast cells. Membranes were labelled the lipophilic styryl dye FM 4-64 and imaged by confocal microscopy. We found that the NIR laser produces an increase of the fluorescence intensity at the location of laser spot. This intensity boost vanishes when the laser is turned off. The time taken by the increase and decrease is of the order of 10 seconds. The mean fluorescence increase, calculated over 75 independent measurements, equals 19%. The experiments reveal that the fluorescence rise is a growing function of the laser power. This dependence is well fitted with a square root function. The NIR laser provokes a rise in the number of molecular associations dye-lipid. The results reported here may be a consequence of a combination of induced increments in membrane fluidity and exocytosis. To the best of our knowledge, this is the first demonstration of the influence of focused NIR laser on the lipid dynamics of a live cell plasma membrane.

Paper Details

Date Published: 1 March 2019
PDF: 8 pages
Proc. SPIE 10876, Optical Interactions with Tissue and Cells XXX, 108760L (1 March 2019); doi: 10.1117/12.2507904
Show Author Affiliations
Remy Avila, Univ. Nacional Autónoma de México (Mexico)
Elisa Tamariz, Instituto de Ciencias de la Salud, Univ. Veracruzana (Mexico)
Norma Medina-Villalobos, Instituto de Ciencias de la Salud, Univ. Veracruzana (Mexico)
Jordi Andilla, ICFO - Institut de Ciències Fotòniques (Spain)
The Barcelona Institute of Science and Technology (Spain)
Maria Marsal, ICFO - Institut de Ciències Fotòniques (Spain)
Pablo Loza-Alvarez, ICFO - Institut de Ciències Fotòniques (Spain)

Published in SPIE Proceedings Vol. 10876:
Optical Interactions with Tissue and Cells XXX
Hope Thomas Beier; Bennett L. Ibey, Editor(s)

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