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Non-invasive monitoring of circulating melanoma cells by in vivo photoacoustic flow cytometry
Author(s): Haiwei Wu; Quanyu Zhou; Huan Zhou; Kai Ping; Bobo Gu; Hao He; Xunbin Wei
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Paper Abstract

Malignant melanoma, developing from melanocytes, is a kind of high metastatic tumor. Circulating melanoma cells, as a marker for metastasis development, are found in blood or lymphatic system at the early stage. Thus, quantitative detection of circulating melanoma cells has great significance to diagnose carcinoma and monitor tumor metastasis. In contrast to in vitro detection methods and in vivo fluorescence-based flow cytometry (IVFC), the in vivo photoacoustic flow cytometry (PAFC) utilizes melanoma cells’ predominant optical absorption in the near-infrared range over other absorbers to receive the photoacoustic (PA) signals without fluorescent dye labeling in a non-invasive way. The sensitivity of the PAFC system was verified by in vitro and in vivo experiments. Besides, we solves the technical problem that blood vessels cannot be positioned in the process of in vivo detection by designing the laser signal positioning navigation system using frequency doubling technique. PAFC provides a new tool for in vivo, label-free, and noninvasive detection of circulating tumor cells (CTCs) and has strong practicality and favorable clinical prospects.

Paper Details

Date Published: 7 March 2019
PDF: 6 pages
Proc. SPIE 10879, Biophotonics and Immune Responses XIV, 108790E (7 March 2019); doi: 10.1117/12.2507807
Show Author Affiliations
Haiwei Wu, Shanghai Jiao Tong Univ. (China)
Quanyu Zhou, Shanghai Jiao Tong Univ. (China)
Huan Zhou, Shanghai Jiao Tong Univ. (China)
Kai Ping, Shanghai Jiao Tong Univ. (China)
Bobo Gu, Shanghai Jiao Tong Univ. (China)
Hao He, Shanghai Jiao Tong Univ. (China)
Xunbin Wei, Shanghai Jiao Tong Univ. (China)


Published in SPIE Proceedings Vol. 10879:
Biophotonics and Immune Responses XIV
Wei R. Chen, Editor(s)

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