Share Email Print
cover

Proceedings Paper • new

Super-resolution multiphoton frequency-domain fluorescence lifetime imaging microscopy by generalized stepwise optical saturation (GSOS)
Author(s): Yide Zhang; David Benirschke; Ola Abdalsalam; Scott S. Howard
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We present the first experimental demonstration of super-resolution multiphoton frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM). This is obtained through a novel microscopy technique called generalized stepwise optical saturation (GSOS). GSOS√utilizes the linear combination of M steps of raw images to improve the imaging resolution by a factor of √M . Here, a super-resolution multiphoton FD-FLIM is demonstrated on various samples, including fixed cells and biological tissues, with a custom-built two-photon FD-FLIM microscope. We demonstrate simultaneous super-resolution intensity and fluorescence lifetime images of a variety of cell cultures and ex vivo tissues. Combined with multiphoton excitation, the proposed GSOS microscopy is able to generate super-resolution FLIM images deep in scattering samples.

Paper Details

Date Published: 22 February 2019
PDF: 10 pages
Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840C (22 February 2019); doi: 10.1117/12.2507663
Show Author Affiliations
Yide Zhang, Univ. of Notre Dame (United States)
David Benirschke, Univ. of Notre Dame (United States)
Ola Abdalsalam, Univ. of Notre Dame (United States)
Scott S. Howard, Univ. of Notre Dame (United States)


Published in SPIE Proceedings Vol. 10884:
Single Molecule Spectroscopy and Superresolution Imaging XII
Zygmunt Karol Gryczynski; Ingo Gregor; Felix Koberling, Editor(s)

© SPIE. Terms of Use
Back to Top