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Development of an electrothermal MEMS mirror based two-photon microscopy probe
Author(s): Liang Zhou; Yanpin Chen; Xiaohong Chen; Yunqi Hao; Jason E. Coleman; Huikai Xie
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Paper Abstract

Two-photon microscopy (TPM) has shown its great impact in studying neuronal activity of live animals. Commercial TPM systems require the animals under study to be restrained under the microscope and the 3D images are obtained typically by using bulky galvo scanning mirrors, which limits the flexibility of animal brain imaging to a large extent. To study freely moving animals, miniaturization of the optical scanning system is the key. In this work, a miniature TPM probe has been developed based on an electrothermal MEMS mirror that can be driven under low voltage. The MEMS mirror has an initial tilt angle after fabrication, and its footprint is 3 mm x 4 mm and reflective mirror plate is aluminumcoated with an equivalent diameter of 2.5 mm. This MEMS probe can be directly adapted to a commercial TPM system. In addition to the MEMS mirror for laser beam scanning, inside the MEMS probe head there are a fixed mirror to fold the laser beam and a high NA polymer lens for focusing. This miniature probe can realize an FOV of 3.5°, or a scanning range of 150 μm. This MEMS probe head is compact with a size of 8 mm x 16 mm, which can be further scaled down.

Paper Details

Date Published: 22 February 2019
PDF: 7 pages
Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088230 (22 February 2019); doi: 10.1117/12.2507655
Show Author Affiliations
Liang Zhou, Univ. of Florida (United States)
Yanpin Chen, Univ. of Florida (United States)
Xiamen Univ. (China)
Xiaohong Chen, Univ. of Florida (United States)
Xiamen Univ. (China)
Yunqi Hao, Univ. of Florida (United States)
Zhengzhou Univ. of Light Industry (China)
Jason E. Coleman, Univ. of Florida (United States)
Huikai Xie, Univ. of Florida (United States)
Beijing Institute of Technology (China)

Published in SPIE Proceedings Vol. 10882:
Multiphoton Microscopy in the Biomedical Sciences XIX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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