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Combined visible light optical coherence microscopy and fluorescence imaging system (Conference Presentation)

Paper Abstract

We implemented a combined visible light optical coherence microscopy (OCM) and fluorescence imaging platform. A supercontinuum light source in combination with a variable filter box (NKT Photonics) provided a spectral range of 425-680 nm. The OCM setup consists of a Michelson interferometer and a custom made spectrometer. Specification measurements were performed and an axial resolution of 0.88 μm in brain tissue was achieved. The transversal resolution was dependent on the objective lenses and varied from 2 to 8 μm. To change from OCM to fluorescence imaging, two mirrors had to be simply flipped into the light beam in the setup. For acquisition of fluorescence images, a photon multiplier tube (PMT) was used to detect light which had passed through a matched combination of emission, dichroic and excitation filters. As a first proof of concept, a fluorescence phantom consisting of curcumin powder mixed with mounting medium was imaged. The OCM images showed the three-dimensional structure of this phantom and specific contrast was gained by fluorescence imaging. As a control case, mounting medium without curcumin powder was imaged and no fluorescence was observed. One hallmark of Alzheimer's disease (AD) is the development of extracellular amyloid-beta plaques in the brain. The three-dimensional structure of these plaques was investigated with micrometer scale resolution using the OCM system. Curcumin can be used to specifically label amyloid-beta deposits. Curcumin stained brain slices of an AD mouse model were imaged and a specific contrast was gained by the fluorescence.

Paper Details

Date Published: 13 March 2019
PDF
Proc. SPIE 10867, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII, 108670W (13 March 2019); doi: 10.1117/12.2507445
Show Author Affiliations
Antonia Lichtenegger, Medizinische Univ. Wien (Austria)
Martina Muck, Medizinische Univ. Wien (Austria)
Danielle J. Harper, Medizinische Univ. Wien (Austria)
Marco Augustin, Medizinische Univ. Wien (Austria)
Pablo Eugui , Medizinische Univ. Wien (Austria)
Matthias Salas, Medizinische Univ. Wien (Austria)
Zoe Neuburger, Medizinische Univ. Wien (Austria)
Christoph K. Hitzenberger, Medizinische Univ. Wien (Austria)
Adelheid Wöhrer, Medizinische Univ. Wien (Austria)
Bernhard Baumann, Medizinische Univ. Wien (Austria)


Published in SPIE Proceedings Vol. 10867:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIII
James G. Fujimoto; Joseph A. Izatt, Editor(s)

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