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Comparison of two- and three-dimensional nuclear to cytoplasm ratios in analyzing lung cancer cell lines
Author(s): Hsu-Cheng Huang; Shu-Jen Chiang; Shu-Han Wen; Pei-Jung Lee; Huei-Wen Chen; Yang-Fang Chen; Chen-Yuan Dong
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Paper Abstract

Histological examination has been the primary imaging modality in the diagnosis of eases such as cancer. However, since cells are three-dimensional in nature, the use of traditional nucleus to cytoplasm ratio (N/C) in two dimensions does not represent their three-dimensional structures. In this study, we used two-photon microscopy to acquired threedimensional images of normal human lung cell line Beas2B, human lung adenocarcinoma CL1-0 and CL1-5 cell lines. We determined N/C ratios in two- and three-dimensions and found that 2D N/C-ratio is more precise than 3D N/C-ratio in discriminating normal and cancer cells.

Paper Details

Date Published: 4 March 2019
PDF: 4 pages
Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108810V (4 March 2019); doi: 10.1117/12.2507400
Show Author Affiliations
Hsu-Cheng Huang, National Taiwan Univ. (Taiwan)
Shu-Jen Chiang, National Taiwan Univ. (Taiwan)
Shu-Han Wen, National Taiwan Univ. (Taiwan)
Pei-Jung Lee, National Taiwan Univ. (Taiwan)
Huei-Wen Chen, National Taiwan Univ. (Taiwan)
Yang-Fang Chen, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 10881:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII
Daniel L. Farkas; Attila Tarnok; James F. Leary, Editor(s)

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