Share Email Print

Proceedings Paper • new

Comparison of two- and three-dimensional nuclear to cytoplasm ratios in analyzing lung cancer cell lines
Author(s): Hsu-Cheng Huang; Shu-Jen Chiang; Shu-Han Wen; Pei-Jung Lee; Huei-Wen Chen; Yang-Fang Chen; Chen-Yuan Dong
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Histological examination has been the primary imaging modality in the diagnosis of eases such as cancer. However, since cells are three-dimensional in nature, the use of traditional nucleus to cytoplasm ratio (N/C) in two dimensions does not represent their three-dimensional structures. In this study, we used two-photon microscopy to acquired threedimensional images of normal human lung cell line Beas2B, human lung adenocarcinoma CL1-0 and CL1-5 cell lines. We determined N/C ratios in two- and three-dimensions and found that 2D N/C-ratio is more precise than 3D N/C-ratio in discriminating normal and cancer cells.

Paper Details

Date Published: 4 March 2019
PDF: 4 pages
Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108810V (4 March 2019); doi: 10.1117/12.2507400
Show Author Affiliations
Hsu-Cheng Huang, National Taiwan Univ. (Taiwan)
Shu-Jen Chiang, National Taiwan Univ. (Taiwan)
Shu-Han Wen, National Taiwan Univ. (Taiwan)
Pei-Jung Lee, National Taiwan Univ. (Taiwan)
Huei-Wen Chen, National Taiwan Univ. (Taiwan)
Yang-Fang Chen, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 10881:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII
Daniel L. Farkas; Attila Tarnok; James F. Leary, Editor(s)

© SPIE. Terms of Use
Back to Top