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Targeted near-infrared fluorescence combined with optical coherence tomography for cancer endoscopic imaging (Conference Presentation)
Author(s): Margherita Vaselli; Fabio Feroldi; Valentina Davidoiu; Johannes de Boer
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Paper Abstract

Early detection of cancer lesions is a critical factor for improving disease prognosis. Detection rates might be improved by catheter based optical coherence tomography (OCT), which enables three dimensional high-resolution images acquisition of organ surfaces and luminal walls. However, OCT lacks in specificity; this limitation can be overcome combining it with targeted fluorescence. To this end, we developed a miniature motorized endoscopic probe with an outer diameter of 1.35mm and a rotation speed of 3,200rpm. Combining near-infrared fluorescence (NIRF) imaging with our OCT system we were able to specifically locate a cell-type by targeting its membrane receptors with fluorescently labelled monoclonal antibodies (mAbs). Exploiting double clad fiber (DCF) coupler technology the OCT and NIRF excitation signals were delivered to the endoscope tip through its single mode core, while the emitted fluorescence signal was efficiently collected by the DCF inner cladding. The catheter was demonstrated to acquire in situ images in a xenograft mouse model of human colorectal cancer, by metabolized mAb labelled with a near-infrared fluorophore (IRDye800CW). While circumferentially scanning the sample with the endoscope, the NIRF signal served as navigation tool to identify the tumour location; once a suspicious region was identified, B-scans and NIRF were acquired. NIRF and OCT images proved to complement each other by revealing molecular contrast within the surrounding tissue architectural context. Moreover, high degree of heterogeneity in the malignant tissue was revealed by the NIRF images. The excellent contrast provided by endoscopic immuno-NIRF-OCT demonstrated its potential to reduce erroneous sampling of tissue.

Paper Details

Date Published: 4 March 2019
Proc. SPIE 10854, Endoscopic Microscopy XIV, 108540E (4 March 2019); doi: 10.1117/12.2507206
Show Author Affiliations
Margherita Vaselli, Vrije Univ. Amsterdam (Netherlands)
Fabio Feroldi, Vrije Univ. Amsterdam (Netherlands)
Valentina Davidoiu, Vrije Univ. Amsterdam (Netherlands)
Johannes de Boer, Vrije Univ. Amsterdam (Netherlands)

Published in SPIE Proceedings Vol. 10854:
Endoscopic Microscopy XIV
Guillermo J. Tearney; Thomas D. Wang; Melissa J. Suter, Editor(s)

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