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Imaging neural activity in zebrafish larvae with adaptive optics and structured illumination light sheet microscopy
Author(s): Yang Liu; Keelan Lawrence; Aqsa Malik; Chelsea E. Gunderson; Rebecca Ball; James D. Lauderdale; Peter Kner
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Paper Abstract

Zebrafish are an important vertebrate model used to view the mechanisms underlying seizure disorders. Due to their relatively small size and transparency, larval zebrafish are an excellent model through which to view the occurence of seizure-like neural activity in vivo using light sheet fluorescence microscopy (LSFM). Although LSFM possesses good optical sectioning capability and high speed, the resolution and contrast degrade as the imaging plane is moved deeper into the sample due to refractive index variations. We have developed a system that combines a structured illumination light sheet microscope with adaptive optics in the emission path to correct optical aberrations and increase the resolution when imaging deep into the sample. We show that our system can record neural activity fast enough to capture seizure events, and is able to correct optical aberrations throughout the sample.

Paper Details

Date Published: 20 February 2019
PDF: 8 pages
Proc. SPIE 10886, Adaptive Optics and Wavefront Control for Biological Systems V, 1088607 (20 February 2019); doi: 10.1117/12.2507048
Show Author Affiliations
Yang Liu, The Univ. of Georgia (United States)
Keelan Lawrence, The Univ. of Georgia (United States)
Aqsa Malik, Emory Univ. (United States)
Chelsea E. Gunderson, The Univ. of Georgia (United States)
Rebecca Ball, The Univ. of Georgia (United States)
James D. Lauderdale, The Univ. of Georgia (United States)
Peter Kner, The Univ. of Georgia (United States)

Published in SPIE Proceedings Vol. 10886:
Adaptive Optics and Wavefront Control for Biological Systems V
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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