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Laser direct-write patterned paper-based devices for detection of bacterial pathogens (Conference Presentation)
Author(s): Peijun He; Ioannis Katis; Catherine Bryant; Charles Keevil; Bhaskar Somani; Robert Eason; Collin Sones

Paper Abstract

Antimicrobial resistance (AMR) has been identified by the World Health Organisation as a global threat that currently claims at least 50,000 lives each year across Europe and the US, with many hundreds of thousands more dying in other areas of the world. The current routine empirical antibiotic therapy protocols that involve laboratory-based bacterial culture testing normally takes up to 2-3 days and are a primary contributor to the global prevalence of AMR. There is therefore an urgent need for low-cost but reliable point-of-care diagnostic solutions for rapid and early screening of infections especially in developing countries that have a lack of both infrastructure and trained personnel. The objective of our research is to fabricate a novel paper-based bacterial culture device that can be used for infection testing even by unskilled users in low resource settings. Here, we present our preliminary results relating to use of our unique laser-patterned paper-based devices for detection and susceptibility testing of E.Coli/Psedomonas, one of the leading causes of urinary tract and upper respiratory tract infections. These paper devices were fabricated via a laser direct-write procedure that uses c.w. laser light at 405nm to cure a photopolymer and produce within paper substrates hydrophobic walls that extend through the thickness of the paper thereby defining separate test zones within the fluidic device. Our laser-patterned paper device has multiple test zones impregnated with agar (containing different antibiotics in various doses) allowing only the selective growth/inhibition and thus detection/susceptibility-testing of E.coli/Pseudomonas via a simple visually observable colour change.

Paper Details

Date Published: 4 March 2019
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Proc. SPIE 10875, Microfluidics, BioMEMS, and Medical Microsystems XVII, 1087519 (4 March 2019); doi: 10.1117/12.2506790
Show Author Affiliations
Peijun He, Univ. of Southampton (United Kingdom)
Ioannis Katis, Univ. of Southampton (United Kingdom)
Catherine Bryant, Univ. of Southampton (United Kingdom)
Charles Keevil, Univ. of Southampton (United Kingdom)
Bhaskar Somani, Univ. Hospital Southampton, NHS Trust (United Kingdom)
Robert Eason, Univ. of Southampton (United Kingdom)
Collin Sones, Univ. of Southampton (United Kingdom)


Published in SPIE Proceedings Vol. 10875:
Microfluidics, BioMEMS, and Medical Microsystems XVII
Bonnie L. Gray; Holger Becker, Editor(s)

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