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Fundamental limits of measuring single-molecule rotational mobility
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Paper Abstract

Various methods exist for measuring molecular orientation, thereby providing insight into biochemical activities at nanoscale. Since fluorescence intensity and not electric field is detected, these methods are limited to measuring even-order moments of molecular orientation. However, any measurement noise, for example photon shot noise, will result in nonzero measurements of any of these even-order moments, thereby causing rotationally-free molecules to appear to be partially constrained. Here, we build a model to quantify measurement errors in rotational mobility. Our theoretical framework enables scientists to choose the optimal single-molecule orientation measurement technique for any desired measurement accuracy and photon budget.

Paper Details

Date Published: 22 February 2019
PDF: 7 pages
Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 1088412 (22 February 2019); doi: 10.1117/12.2506712
Show Author Affiliations
Oumeng Zhang, Washington Univ. in St. Louis (United States)
Matthew D. Lew, Washington Univ. in St. Louis (United States)

Published in SPIE Proceedings Vol. 10884:
Single Molecule Spectroscopy and Superresolution Imaging XII
Zygmunt Karol Gryczynski; Ingo Gregor; Felix Koberling, Editor(s)

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