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Proceedings Paper

Esophageal cancer detection based on two-photon excitation fluorescence combined with membrane electrophoresis of blood serum
Author(s): Nenrong Liu; Weisheng Chen; Xianzeng Zhang; Rong Chen
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Paper Abstract

Nonlinear optical spectroscopy has wide applications in the medical field with special advantages. Through combining nonlinear optical spectroscopy with membrane electrophoresis, we endeavored to develop a novel method for blood serum analysis for cancer detection applications. In this method, albumin and globin are isolated from blood serum by membrane electrophoresis to perform two-photon excitation fluorescences (TPEF) spectral analysis. The obtained spectra present rich signatures of the biochemical constituents of whole proteins. We evaluated the utility of this method by analyzing albumin and globin samples of blood serum from esophageal cancer patients and healthy volunteers. Twophoton excitation fluorescences revealed that esophageal cancer group can be unambiguously discriminated from the normal group, and I457/I639, I511/I639, and I543/I639 ratios can be used as indicators to diagnose early esophageal cancer. These results are very promising for developing a label-free, non-invasive clinical tool for early cancer detection and screening.

Paper Details

Date Published: 23 October 2018
PDF: 7 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108203J (23 October 2018); doi: 10.1117/12.2502720
Show Author Affiliations
Nenrong Liu, Fujian Normal Univ. (China)
Weisheng Chen, Fujian Medical Univ. (China)
Xianzeng Zhang, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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