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Proceedings Paper

Colorimetric system for paper-based assays
Author(s): Demetre Liakos; Suzanne Smith; Trudi-Heleen Joubert
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Paper Abstract

Paper-based biosensors are used in the field for their low cost and disposability. Their results are often Boolean, however quantitative results would improve derived outcomes. Devices exist which use colorimetry or spectroscopy to extract this data, however they are often bulky, expensive, and specific to the type of assay being performed. The aim of this project was to design a low-cost, portable, and easy to use image-sensing device to optically capture and log results from activated paper-based assays. Markings printed on the assays aid with detection of the assay type. This provides a system extensible to assays of various shapes and sizes. New assays can easily be created and uploaded to the device. Measurements can be taken from up to 30 cm away from the sensor. Once captured, the reaction areas are identified, and the colour intensities are measured to a resolution of 7 bits. Calibration curves are then used to convert these intensities to appropriate units.

Paper Details

Date Published: 24 January 2019
PDF: 6 pages
Proc. SPIE 11043, Fifth Conference on Sensors, MEMS, and Electro-Optic Systems, 110430J (24 January 2019); doi: 10.1117/12.2502538
Show Author Affiliations
Demetre Liakos, Univ. of Pretoria (South Africa)
Suzanne Smith, Council for Scientific and Industrial Research (South Africa)
Trudi-Heleen Joubert, Univ. of Pretoria (South Africa)

Published in SPIE Proceedings Vol. 11043:
Fifth Conference on Sensors, MEMS, and Electro-Optic Systems
Monuko du Plessis, Editor(s)

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