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The construction of genomic libraries in BAC and its practical application and bioinformatic usage
Author(s): Agnieszka Skarzyńska; Kohei Yagi; Maciej Kiełek; Wojciech Gutman; Wojciech Pląder; Zbigniew Przybecki
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Paper Abstract

Libraries constructed in bacterial artificial chromosome (BAC) vectors are essential in modern genomics for all organisms such as plants, animals, insects, algae and microbes. Cloning using a bacterial artificial chromosome (BAC) allows to obtain high quality genomic libraries that are used for physical mapping, cytogenetic research, identification and isolation of genes, as well as for gene sequencing and genomic assembly. Here we describe the methodology of BAC library construction, characterization and the newest application and bioinformatics usage in the post-genomic era.

Paper Details

Date Published: 1 October 2018
PDF: 10 pages
Proc. SPIE 10808, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2018, 1080835 (1 October 2018); doi: 10.1117/12.2501371
Show Author Affiliations
Agnieszka Skarzyńska, Warsaw Univ. of Life Sciences (Poland)
Kohei Yagi, Warsaw Univ. of Life Sciences (Poland)
Maciej Kiełek, Warsaw Univ. of Life Sciences (Poland)
Wojciech Gutman, Warsaw Univ. of Life Sciences (Poland)
Wojciech Pląder, Warsaw Univ. of Life Sciences (Poland)
Zbigniew Przybecki, Warsaw Univ. of Life Sciences (Poland)


Published in SPIE Proceedings Vol. 10808:
Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2018
Ryszard S. Romaniuk; Maciej Linczuk, Editor(s)

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