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Spatiotemporal dynamics of molecular messaging in bacterial co-cultures studied by multimodal chemical imaging
Author(s): Tianyuan Cao; Nydia Morales-Soto; Jin Jia; Nameera F. Baig; Sage J. B. Dunham; Joseph Ellis; Jonathan V. Sweedler; Joshua D. Shrout; Paul W. Bohn
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Paper Abstract

Microbial community behavior is coupled to a set of genetically-regulated chemical signals that correlate with cell density – the quorum sensing (QS) system – and there is growing appreciation that the QS-regulated behavior of bacteria is chemically, spatially, and temporally complex. In addition, while it has been known for some time that different species use different QS networks, we are beginning to appreciate that different strains of the same bacterial species also differ in their QS networks. Here, we combine mass spectrometric imaging (MSI) and confocal Raman microscopy (CRM) approaches to investigate co-cultures involving different strains (FRD1 and PAO1C) of the same species (Pseudomonas aeruginosa) as well as those involving different species (P. aeruginosa and E. coli). Combining MSI and CRM makes it possible to supersede the limits imposed by individual imaging approaches and enables the spatial mapping of individual bacterial species and their microbial products within a mixed bacterial community growing in situ on surfaces. MSI is used to delineate the secretion of a specific rhamnolipid surfactant as well as alkyl quinolone (AQ) messengers between FRD1 and PAO1C strains of P. aeruginosa, showing that the spatial distribution and production of AQ messengers in PAO1C differs substantially from that of FRD1. In the case of multiple species, CRM is used to show that the prolific secretion of AQs by the PAO1C strain of P. aeruginosa is used to mediate its interaction with cocultured E. coli.

Paper Details

Date Published: 7 March 2019
PDF: 10 pages
Proc. SPIE 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 108630A (7 March 2019); doi: 10.1117/12.2501349
Show Author Affiliations
Tianyuan Cao, Univ. of Notre Dame (United States)
Nydia Morales-Soto, Univ. of Notre Dame (United States)
Jin Jia, Univ. of Notre Dame (United States)
Nameera F. Baig, Lawrence Berkeley National Lab. (United States)
Sage J. B. Dunham, Entech Instruments (United States)
Univ. of Illinois at Urbana-Champaign (United States)
Joseph Ellis, Univ. of Illinois (United States)
Jonathan V. Sweedler, Univ. of Illinois (United States)
Joshua D. Shrout, Univ. of Notre Dame (United States)
Paul W. Bohn, Univ. of Notre Dame (United States)

Published in SPIE Proceedings Vol. 10863:
Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II
Tianhong Dai; Jürgen Popp; Mei X. Wu, Editor(s)

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