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Proceedings Paper

Characterization of the germination kinetics of individual Nosema bombycis spores using phase contrast microscopy imaging and Raman spectroscopy
Author(s): Zhenbin Miao; Yu Zhang; Pengfei Zhang; Xiaochun Wang; Junxian Liu; Guiwen Wang
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Paper Abstract

We used phase-contrast microscopy and laser tweezers Raman spectroscopy to monitor the changes of intracellular components during the spore germination of microsporidian Nosema bombycis under artificial conditions. The kinetics of image brightness of individual spores showed three-phase profile. The Raman intensities of trehalose, nucleic acid, and protein showed little changes prior to germination, but dramatically decreased once germinated, which was consistent with the kinetics of brightness of the spore. The time of spore germination (ΔTgerm) was essentially identical, but the initial time of germination (Tlag) showed obvious heterogeneity. These results demonstrate that the firing of the polar tube is not performed via trehalose transformation to the spore’s osmosis during germination. Phase-contrast microscopy imaging and Raman spectroscopy provide a more comprehensive approach to understanding the germination of microsporidian spores.

Paper Details

Date Published: 23 October 2018
PDF: 7 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108202Z (23 October 2018); doi: 10.1117/12.2501212
Show Author Affiliations
Zhenbin Miao, Guangxi Academy of Sciences (China)
Yu Zhang, Guangxi Academy of Sciences (China)
Guangxi Normal Univ. (China)
Pengfei Zhang, Tianjin Univ. (China)
Xiaochun Wang, Guangxi Academy of Sciences (China)
Junxian Liu, Guangxi Normal Univ. (China)
Guiwen Wang, Guangxi Academy of Sciences (China)

Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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