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Effcient quantitative phase imaging for programmable LED light microscopy
Author(s): Jiaji Li; Jialin Zhang; Qian Chen; Chao Zuo
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Paper Abstract

We demonstrate an efficient quantitative phase imaging (QPI) approach using programmable annular LED illumination. As a new type of coded light source, the LED array provides flexible illumination control for noninterferometric QPI based on a traditional microscopic configuration. The proposed annular LED illumination modulates the transfer function of system by changing the LED illumination pattern, which provides noiserobust response of transfer function and achieves twice resolution limit of objective NA. The quantitative phase can be recovered from slightly defocused intensity images through inversion of transfer function. Moreover, the weak object transfer function (WOTF) of axis-symmetric oblique source is derived, and the noise-free and noisy simulation results validate the predicted theory. Finally, we experimentally confirm accurate and repeatable performance of our method by imaging cellular specimens with different NA objectives.

Paper Details

Date Published: 24 July 2018
PDF: 5 pages
Proc. SPIE 10827, Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018), 108273P (24 July 2018); doi: 10.1117/12.2500975
Show Author Affiliations
Jiaji Li, Nanjing Univ. of Science and Technology (China)
Jialin Zhang, Nanjing Univ. of Science and Technology (China)
Qian Chen, Nanjing Univ. of Science and Technology (China)
Chao Zuo, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 10827:
Sixth International Conference on Optical and Photonic Engineering (icOPEN 2018)
Yingjie Yu; Chao Zuo; Kemao Qian, Editor(s)

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