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Proceedings Paper

STED super-resolution imaging of mitochondria in live cells
Author(s): Luwei Wang; Wei Yan; Zhigang Yang; Junle Qu II
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Paper Abstract

A series of new fluorescent probes were developed to carry out live cell super-resolution imaging with low STED laser power or suitable STORM working conditions. And STED-FLIM imaging of microtubules labeled with ATTO647N inside HeLa cells and the mitosis process was obtained, which provides new insight into the cell structure and functions.Finally, stochastic optical reconstruction microscopy (STORM) super-resolution imaging of mitochondrial membrane in live HeLa cells was obtained by the implementation of new fluorescent probes, improved imaging system and optimized single molecule localization algorithm. This provided an important tool and strategy for studying dynamic events and complex functions in living cells.

Paper Details

Date Published: 23 October 2018
PDF: 1 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201Y (23 October 2018); doi: 10.1117/12.2500716
Show Author Affiliations
Luwei Wang, Shenzhen Univ. (China)
Wei Yan, Shenzhen Univ. (China)
Zhigang Yang, Shenzhen Univ. (China)
Junle Qu II, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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