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Proceedings Paper

Label-free multiphoton microscopy for ex vivo brain imaging: toward assisting pathologic diagnosis
Author(s): Shu Wang; Airong Liu; Feng Wang; Ruolan Lin; Yunkun Ma; Bingbing Lin; Yue Chen; Zhong Chen; Xiaoqin Zhu; Yuanxiang Lin; Xingfu Wang; Jianxin Chen
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Paper Abstract

Standard histopathology is well accepted as the gold standard for the diagnosis a wide range of diseases. Despite continuing advances in tissue staining automation, typical histological processing such as formalin-fixed paraffin-embedded are also labour- and time-intensive for treatment decisions in intraoperative histopathologic diagnosis. Multiphoton microscopy (MPM), based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), can be a versatile tool that enables label-free mapping of endogenous fluorophores within a fresh specimen, which provides pathology-like images with cellular and subcellular details. Here, we describe the use of label-free MPM for visualizing rat and human ex vivo brain tissue without tissue fixation, processing, and staining. Moreover, MPM is able to identify 6 types of cells in rat cerebrum and cerebellum, including cortical neurons, glia cells, Purkinje cells, pyramidal neurons and granule neurons in hippocampus, as well as epithelial cells in lateral ventricle. In addition, we further demonstrate that MPM can provide definitive pathological features in cerebral ischemia and focal cortical dysplasia (FCD) for assisting pathologic diagnosis. Our work establishes the methodology and augments the diagnostic accuracy of traditional frozen section histopathology. With the development of the miniature two-photon microscope, MPM will show more potential as a practical clinical tool for providing intraoperative reference image guidance of resection in neurosurgery.

Paper Details

Date Published: 23 October 2018
PDF: 8 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201X (23 October 2018); doi: 10.1117/12.2500715
Show Author Affiliations
Shu Wang, Fujian Normal Univ. (China)
Airong Liu, Yantai Engineering & Technology College (China)
Feng Wang, The First Affiliated Hospital of Fujian Medical Univ. (China)
Ruolan Lin, Fujian Medical Univ. Union Hospital (China)
Yunkun Ma, National Univ. of Singapore (Singapore)
Bingbing Lin, Fujian Univ. of Traditional Chinese Medicine (China)
Yue Chen, The First Affiliated Hospital of Fujian Medical Univ. (China)
Zhong Chen, Fujian Normal Univ. (China)
Xiaoqin Zhu, Fujian Normal Univ. (China)
Yuanxiang Lin, The First Affiliated Hospital of Fujian Medical Univ. (China)
Xingfu Wang, The First Affiliated Hospital of Fujian Medical Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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