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Proceedings Paper

Quantitative FRET measurement of the interaction of A1 adenosine receptors and A2A adenosine receptors in living cell
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Paper Abstract

Adenosine plays important roles in the pain signal transduction by activating adenosine receptors of two subtypes of A1 and A2A. In this study, FRET system based independent emission -spectral spectral spectral unmixing method (Iem-spFRET) was set up and used to measure the energy transfer from A1R to A2AR. The energy transfer efficiency calculated by Iem-spFRET is about 17.44%. All the above date and results demonstrate that FRET with special designed fluorescence proteins could be used to investigate the interaction between adenosine receptors.

Paper Details

Date Published: 23 October 2018
PDF: 6 pages
Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201V (23 October 2018); doi: 10.1117/12.2500692
Show Author Affiliations
Yating Lin, Fujian Normal Univ. (China)
Yiming Huang, Fujian Normal Univ. (China)
Zhiwei Wu, Fujian Normal Univ. (China)
Jianshu Xu, Fujian Normal Univ. (China)
Ya Hu, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Juqiang Lin, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 10820:
Optics in Health Care and Biomedical Optics VIII
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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